bio-reverse-complement

Generate complementary and reverse complementary sequences using Biopython. from Bio.Seq import Seq Returns the reverse ...

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bio-restriction-enzyme-selection

from Bio import SeqIO from Bio.Restriction import Analysis, CommOnly, AllEnzymes record = SeqIO.read('sequence.fasta', '...

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bio-research-tools-biomarker-signature-studio

Design validated biomarker panels that are explainable, stable, and ready for translational follow-up. This skill stitch...

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bio-reporting-rmarkdown-reports

--- title: "RNA-seq Analysis Report" author: "Your Name" date: "r Sys.Date()" output: htmldocument: toc: true tocfloat: ...

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bio-reporting-quarto-reports

--- title: "Analysis Report" author: "Your Name" date: today format: html: toc: true code-fold: true theme: cosmo --- --...

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bio-reporting-figure-export

import matplotlib.pyplot as plt plt.rcParams.update({ 'font.size': 8, 'font.family': 'Arial', 'axes.linewidth': 0.5, 'li...

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bio-read-sequences

Reference examples tested with: BioPython 1.83+ Before using code patterns, verify installed versions match. If versions...

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bio-read-qc-umi-processing

Reference examples tested with: pandas 2.2+, samtools 1.19+ Before using code patterns, verify installed versions match....

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bio-read-qc-quality-filtering

Reference examples tested with: Trimmomatic 0.39+, cutadapt 4.4+, fastp 0.23+ Before using code patterns, verify install...

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bio-read-alignment-star-alignment

STAR --runMode genomeGenerate \ --runThreadN 8 \ --genomeDir starindex/ \ --genomeFastaFiles reference.fa \ --sjdbGTFfil...

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bio-read-alignment-bowtie2-alignment

bowtie2-build reference.fa referenceindex bowtie2-build --threads 8 reference.fa referenceindex bowtie2 -p 8 -x referenc...

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bio-reaction-enumeration

Reference examples tested with: RDKit 2024.03+ Before using code patterns, verify installed versions match. If versions ...

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bio-proteomics-quantification

Reference examples tested with: MSnbase 2.28+, numpy 1.26+, pandas 2.2+ Before using code patterns, verify installed ver...

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bio-proteomics-protein-inference

Reference examples tested with: pyOpenMS 3.1+ Before using code patterns, verify installed versions match. If versions d...

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bio-proteomics-differential-abundance

Reference examples tested with: R stats (base), ggplot2 3.5+, limma 3.58+, numpy 1.26+, pandas 2.2+, scipy 1.12+, statsm...

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bio-proteomics-dia-analysis

Reference examples tested with: numpy 1.26+, pandas 2.2+ Before using code patterns, verify installed versions match. If...

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bio-primer-design-qpcr-primers

Design primers and internal probes for quantitative PCR using primer3-py. import primer3 from Bio import SeqIO sequence ...

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bio-primer-design-primer-validation

Check primers for secondary structures, dimers, and other issues using primer3-py. import primer3 primer = 'ATGCGATCGATC...

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bio-population-genetics-selection-statistics

Detect natural selection signatures using diversity statistics and extended haplotype homozygosity. import allel import ...

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bio-population-genetics-population-structure

Analyze genetic ancestry and population stratification using PCA and ADMIXTURE. plink2 --bfile data --pca 10 --out pcare...

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bio-population-genetics-linkage-disequilibrium

Calculate LD statistics, prune correlated variants, and identify haplotype blocks. plink2 --bfile data --r2 --ld-window-...

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bio-population-genetics-association-testing

GWAS analysis using PLINK 2.0's unified --glm command for case-control and quantitative traits. plink2 --bfile data --gl...

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bio-pileup-generation

Generate pileup data for variant calling and position-level analysis. Pileup shows all reads covering each position in t...

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bio-phylo-tree-io

Parse, write, and convert phylogenetic tree files in various formats. from Bio import Phylo from io import StringIO | Fo...

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