bio-hi-c-analysis-hic-differential

Reference examples tested with: cooler 0.9+, cooltools 0.6+, matplotlib 3.8+, numpy 1.26+, pandas 2.2+, scipy 1.12+, sta...

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bio-hi-c-analysis-hic-data-io

Reference examples tested with: cooler 0.9+, numpy 1.26+, pandas 2.2+, scanpy 1.10+, scipy 1.12+ Before using code patte...

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bio-hi-c-analysis-contact-pairs

Reference examples tested with: cooler 0.9+, pairtools 1.1+, pandas 2.2+ Before using code patterns, verify installed ve...

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bio-hi-c-analysis-compartment-analysis

Reference examples tested with: cooler 0.9+, cooltools 0.6+, matplotlib 3.8+, numpy 1.26+, pandas 2.2+, scipy 1.12+ Befo...

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bio-genome-intervals-proximity-operations

Operations for finding nearby features and extending intervals using bedtools and pybedtools. bedtools closest -a peaks....

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bio-genome-intervals-gtf-gff-handling

GTF and GFF3 are standard gene annotation formats. Both use 1-based coordinates. | Feature | GTF | GFF3 | |---------|---...

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bio-genome-engineering-off-target-prediction

Reference examples tested with: pandas 2.2+ Before using code patterns, verify installed versions match. If versions dif...

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bio-genome-assembly-scaffolding

bwa index draftassembly.fa bwa mem -5SP -t 16 draftassembly.fa hicR1.fq.gz hicR2.fq.gz | \ samtools view -@ 8 -bhS - > a...

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bio-genome-assembly-metagenome-assembly

Metagenome assembly reconstructs genomes from mixed microbial communities. Long reads enable recovery of complete circul...

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bio-genome-assembly-hifi-assembly

hifiasm -o outputprefix -t 32 reads.hifi.fastq.gz awk '/^S/{print ">"$2;print $3}' outputprefix.bp.pctg.gfa > assembly.f...

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bio-genome-assembly-contamination-detection

checkm2 predict --input assembly.fa --output-directory checkm2output --threads 16 checkm2 predict --input genomes/ --out...

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bio-format-conversion

Reference examples tested with: BioPython 1.83+, samtools 1.19+ Before using code patterns, verify installed versions ma...

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bio-flow-cytometry-gating-analysis

Reference examples tested with: flowCore 2.14+ Before using code patterns, verify installed versions match. If versions ...

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bio-flow-cytometry-fcs-handling

Reference examples tested with: flowCore 2.14+, scanpy 1.10+ Before using code patterns, verify installed versions match...

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bio-flow-cytometry-doublet-detection

Reference examples tested with: flowCore 2.14+, ggplot2 3.5+ Before using code patterns, verify installed versions match...

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bio-flow-cytometry-cytometry-qc

Reference examples tested with: flowCore 2.14+, ggplot2 3.5+ Before using code patterns, verify installed versions match...

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bio-flow-cytometry-bead-normalization

Reference examples tested with: flowCore 2.14+, ggplot2 3.5+ Before using code patterns, verify installed versions match...

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bio-expression-matrix-gene-id-mapping

import mygene import pandas as pd mg = mygene.MyGeneInfo() ensemblids = ['ENSG00000141510', 'ENSG00000012048', 'ENSG0000...

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bio-expression-matrix-counts-ingest

import pandas as pd counts = pd.readcsv('counts.tsv', sep='\t', indexcol=0) counts = pd.readcsv('counts.csv', indexcol=0...

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bio-experimental-design-power-analysis

Power = probability of detecting a true effect. Underpowered studies waste resources; overpowered studies are inefficien...

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bio-experimental-design-multiple-testing

Testing 20,000 genes at p < 0.05 yields 1,000 false positives by chance. Correction is essential. padj <- p.adjust(pvalu...

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bio-experimental-design-batch-design

Batch effects are unavoidable. Good design makes them correctable. Never confound batch with condition - Each batch must...

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bio-epitranscriptomics-modification-visualization

library(Guitar) library(TxDb.Hsapiens.UCSC.hg38.knownGene) peaks <- import('m6apeaks.bed') GuitarPlot( peaks, txdb = TxD...

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bio-epitranscriptomics-m6a-peak-calling

library(exomePeak2) result <- exomePeak2( bamip = c('IPrep1.bam', 'IPrep2.bam'), baminput = c('Inputrep1.bam', 'Inputrep...

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